PCR, Cloning and Recombinant Technology...

Just something to digest. Haha.. Enjoy!

Monday Morning......

Salam..

Just a quick update.. Hehe.. Its been 2 weeks since I've started 'working' and its been relatively quiet.. Just some funny accidents, and miscommunications, added with some reading-up on my part, I can safely say things are beginning to become a routine.

Come in at 8.30 straight to the lab, open up laptop, taking the necessary things outta my bag, read my gene cloning book, going online, facebooking, checking out mysoju for latest episodes (or old ones, depending on my mood), waiting for others to come, getting bored, jalan-jalan mengacau orang, waiting for lunch time, lunch, surau, back to the lab, online again, read again, then walla! its 5.30.. Go back..

Haizzz.... Oh well, things should be picking up soon.. The routine will supposedly be broken today coz I'll be going to FST this afternoon to meet my guide.. Will get the gene template from her and her friend will teach me how to design primers. Hopefully, I will be able to design my forward and backward primer a.s.a.p, so that I could start with PCR..

My terribly cluttered desk, just the way I like it!! =)

Oh well, last week has been full of me getting headaches and questioning if this is what I truly wanted.. There's so many choices, and frankly we are spoiled by 'em, co much that sometimes whatt held us back is just the comfort of things familiar to us..

Entah la.. Berfikir, berfikir dan berfikir... Just hoping that the best choice has been taken.. =)

Wish me luck, people.. And thanks for reading!

Quoting Lady GaGa, "There's Nothing Else I Can Say, Eh Eh....."

Salam..

It's been a week since I started being a research assistant for my lecturer.. It has been mostly uneventful, except for that one time when I had to attend the first meeting for the project group. To tell you the truth, I was freaking out that time because that was the first time I got to know a lot of what I am going to do involves genetic engineering.

Me + Biochemical engineering major + No experience in genetic engineering = ()*&^*(&%$##@@

Haha.. My mind is whirling and swirling with a lot of things while the presentation is being done, mostly because at that time I couldn't understand half of what is being presented! Gosh, how am I going to do that?

Uh oh..

No background, ok, I could read up on DNA, cloning, recombinant technology, PCR, codon, promoters, plasmid, primer, genetic templates so on and so forth, but without any technical experience in this matter, I'm seriously hyper-ventilating!

Huhu.. Its so very challenging for me, and right now I'm still reading up stuffs on genetic engineering.. And at the same time, contacting the various people that I hope could shed some light on all these new things that I will be venturing into.

Please wish me all d best, people.. I'm really interested in doing this, because it's something new.. But dealing with DNA also involves a lot of luck. One colleague of mine told me that she took 27 months to do her research on this particular gene, and that is more than 2 years! Furthermore, for me, not only will I do the genetics part, but I will also continue from that to fermentation stage, and not only that, I will also do the purification!

Oooohhh... Very,very nervous now..

Anyone with PCR expertise?? Call me!

Seriously though, I hope that I could pick up the technical aspects of PCR and cloning, coz even though I somewhat understand the theory, I have no idea on how to achieve that, lab-wise. That is the biggest challenge for me at this moment.

Oh well, no use dwelling too much on the cons of this, I know... I'm trying to look at the bright side.. =)

Again, wish me luck!

Muche gracias..
=)